Visualization of G Protein−Coupled Receptor (GPCR) Interactions in Living Cells Using Bimolecular Fluorescence Complementation (BiFC)
نویسندگان
چکیده
منابع مشابه
Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells.
Protein interactions are a fundamental mechanism for the generation of biological regulatory specificity. The study of protein interactions in living cells is of particular significance because the interactions that occur in a particular cell depend on the full complement of proteins present in the cell and the external stimuli that influence the cell. Bimolecular fluorescence complementation (...
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We present a high-throughput approach to study weak protein-protein interactions by coupling bimolecular fluorescent complementation (BiFC) to flow cytometry (FC). Bimolecular Fluorescence Complementation (BIFC) is a method to detect proteinprotein interactions based on the formation of a fluorescent complex by fragments of the yellow fluorescent protein (NYFP and CYFP) brought together by the ...
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ژورنال
عنوان ژورنال: Current Protocols in Neuroscience
سال: 2010
ISSN: 1934-8584,1934-8576
DOI: 10.1002/0471142301.ns0529s51